Proposal of potentially stabilizing mutations

Protposer is a protein stabilization tool that analyses PDB files and sequence databases in search for wild type amino acid residues providing opportunities for thermostabilization. Protposer proposes a list of point mutations that are likely to increase protein stability by at least 0.5 kcal/mol. To do so, it searches for divergences from ancestral or consensus sequences, from ideal alpha-helix composition or average solvent exposure of polar or apolar residues, and for the presence of cavities, electrostatic imbalance or acidic H-bonds. Protposer finds weak spots in the structure, generates and evaluates replacements, and estimates their probability of being stabilizing, using a machine learning logistic regression model.

Protposer users can indicate a PDB or Alphafold ID code or upload their own PDB file. The results are shown in an HTML summary, containing the proposed mutations, the reason why they are proposed, and how they scored for different features.

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Protposer process
Complete with a PDB or AlphaFold ID code, or Upload a PDB file
Title for this job
Input type
Select Chain

e.g. 1flv Introduce a valid PDB ID code

e.g. A

e.g. AF-P0A3E0-F1 Introduce a valid AF ID code

e.g. A

e.g. mystructure.pdb

e.g. A
Email address to return results
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  • Job id:
  • Job title:
  • PDB:
  • Chain:
  • Email:

Your request will be serviced as soon as possible. Calculations may take from minutes to a few days for an average protein folding domain, depending on the number of queued requests on the server, the size of the submitted protein and the size of its homologous family.

Once your request has been completely serviced, or if there is any problem that does not allow your request to be serviced, you will receive an email with either the results attached or information about the error.

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PDC2021-121341-I00 financiado por MCIN/AEI/10.13039/501100011033 y por la Unión Europea "NextGenerationEU"/PRTR.